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Proceedings Paper

In vivo detection of cervical dysplasia with near-infrared Raman spectroscopy
Author(s): Amy Robichaux; Chad A. Lieber; Heidi Shappell M.D.; Beth Huff; Howard Jones III; Anita Mahadevan-Jansen
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Paper Abstract

Raman spectroscopy has been shown to have the potential for providing differential diagnosis in the cervix with high sensitivity and specificity in previous in vitro and in vivo studies. A clinical study was designed at Vanderbilt University Medical Center to further evaluate the potential of near IR Raman spectroscopy for in vivo detection of squamous intra-epithelial neoplasia, a pre-cursor to cervical cancer, in a clinical setting. In this pilot in vivo clinical study, using a portable system, Raman spectra are collected using clinically feasible integration times during colposcopic evaluation. Multiple Raman spectra were acquired form colposcopically normal and abnormal sites prior to excision of tissue from patients with known abnormalities of the cervix. Measured Raman spectra were processed for nosie and background fluorescence using novel signal processing techniques. The resulting spectra were correlated with the corresponding histological diagnosis to determine empirical differences in spectra between different diagnostic categories. Using histology as the gold standard, multivariate statistical techniques were also used to develop discrimination algorithms with the hopes of developing this technique into a real time, non-invasive diagnostic tool.

Paper Details

Date Published: 27 March 2002
PDF: 7 pages
Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); doi: 10.1117/12.460793
Show Author Affiliations
Amy Robichaux, Vanderbilt Univ. (United States)
Chad A. Lieber, Vanderbilt Univ. (United States)
Heidi Shappell M.D., Vanderbilt Univ. Medical Ctr. (United States)
Beth Huff, Vanderbilt Univ. Medical Ctr. (United States)
Howard Jones III, Vanderbilt Univ. Medical Ctr. (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 4614:
Biomedical Vibrational Spectroscopy II
Anita Mahadevan-Jansen; Henry H. Mantsch; Gerwin J. Puppels, Editor(s)

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