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Proceedings Paper

Comparing FTIR and RAPD techniques in the typing of c. albicans in a clinical set-up
Author(s): Christophe L. Sandt; Ganesh D. Sockalingum; Dominique Toubas; Dominique Aubert; Herve Lepan; Claire Lepouse; Maryse Jaussaud; Alain Leon; Jean-Michel Pinon; Michel Manfait
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Paper Abstract

Candida albicans is an opportunistic pathogen, generally though to be of endogenous origin, with however reported outbreaks. Epidemilogy of C. albicans has been studied so far by genotypic methods mainly, including the classical RAPD analysis. Albeit powerful, genotypic techniques are expensive, time consuming and complex to implement. FTIR spectroscopy is simple, rapid, inexpensive and an increasingly used technique for the identification of microorganisms. As a phenotypic method, it provides rapid whole cells 'fingerprinting' using few consumables and can detect very subtle differences between strains of the same species. In this study, C. albicans strains isolated from 50 patients from six hospital units were collected and studied by FTIR spectroscopy and RAPD-PCR. Discrimination of strains was computed using classification algorithms on selected features of the spectral data. Results from 10 patients, for whom iterative sampling was possible, are presented and discussed. Emphasis was laid on the reproducibility of dat for strain-level identification. FTIR analysis shows that (a) the C. albicans spectra were different from one patient to another, (b) seven patients exhibit each a homogeneous group while three patients display each two groups of strains. RAPD-PCR and FTIR analyses correlate quite well showing that FTIR spectroscopy could be a potential epidemiological tool in the control of nosocomial fungal infections.

Paper Details

Date Published: 27 March 2002
PDF: 11 pages
Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); doi: 10.1117/12.460786
Show Author Affiliations
Christophe L. Sandt, Univ. de Reims Champagne-Ardenne (France)
Ganesh D. Sockalingum, Univ. de Reims Champagne-Ardenne (France)
Dominique Toubas, Univ. de Reims Champagne-Ardenne (France)
Dominique Aubert, Univ. de Reims Champagne-Ardenne (France)
Herve Lepan, Univ. de Reims Champagne-Ardenne (France)
Claire Lepouse, Univ. de Reims Champagne-Ardenne (France)
Maryse Jaussaud, Univ. de Reims Champagne-Ardenne (France)
Alain Leon, Univ. de Reims Champagne-Ardenne (France)
Jean-Michel Pinon, Univ. de Reims Champagne-Ardenne (France)
Michel Manfait, Univ. de Reims Champagne-Ardenne (France)

Published in SPIE Proceedings Vol. 4614:
Biomedical Vibrational Spectroscopy II
Anita Mahadevan-Jansen; Henry H. Mantsch; Gerwin J. Puppels, Editor(s)

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