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Proceedings Paper

Applications of FT-IR spectrometry to plasma contents analysis and monitoring
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Paper Abstract

We devoted efforts to develop an analytical method of plasma able to provide both a high sensitivity and a global overview of its biomolecular contents along with the variations of these ones. Among candidates, transmittance FT-IR spectrometry has proved to be highly efficient. It has been used to analyze plasma micro samples using an iterative process. Results in accordance with clinical data were obtained from a single FT-IR spectrum for the following biomolecules: amino-acids, fatty acids, albumin, glucose, fibrinogen, lactate, triglycerides, glycerol, urea, (alpha) 1-antitrypsin, alpha2-macroglobulin, transferin, Apo-A1, Apo-B, Apo-C3, IgA, IgD, IgG1, IgG2, IgG3, IgG4, IgM, haptoglobin, and (alpha) 1-acid glycoprotein. Moreover, cholesterol contribution may be determined on the same IR spectrum. Therefore, as only micro samples are necessary, high frequency blood analysis become available. This method was used to monitor inflammatory processes related to given metabolic stresses. Moreover, FT-IR spectrum constitutes a 'metabolic photography' of the subject, allowing classification between metabolic groups (pathologic or others). It was used on difference spectra in order to raise 'signal to noise' ratio by elimination of the unvarying spectral contribution. Among others, it allowed to uncover overtraining in high-level sportsmen several weeks before any physiologic or clinical symptom occurred.

Paper Details

Date Published: 27 March 2002
PDF: 9 pages
Proc. SPIE 4614, Biomedical Vibrational Spectroscopy II, (27 March 2002); doi: 10.1117/12.460782
Show Author Affiliations
Gerard Yves Rene Deleris, Univ. Victor Segalen Bordeaux II (France)
Cyril Petibois, Univ. Victor Segalen Bordeaux II (France)

Published in SPIE Proceedings Vol. 4614:
Biomedical Vibrational Spectroscopy II
Anita Mahadevan-Jansen; Henry H. Mantsch; Gerwin J. Puppels, Editor(s)

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