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Proceedings Paper

Optical imaging fiber-based recombinant bacterial biosensor
Author(s): Israel Biran; David M. Rissin; David R. Walt
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Paper Abstract

A cell array biosensor was developed, composed of thousands of individual bacteria cells expressing a reporter gene that responds to the presence of environmental pollutants. The single cell array was produced by immobilizing the cells on an optical imaging fiber. A high-density array of microwells was fabricated on the optical imaging fiber's distal face by selectively etching the individual fibers cores. Each microwell was used to accommodate a single living bacterium, allowing simultaneous monitoring of the genetic responses of all the cells in the array. The optical imaging fiber cell array platform provides a powerful tool for the fabrication of whole cell biosensors for various environmental and industrial applications.

Paper Details

Date Published: 22 February 2002
PDF: 7 pages
Proc. SPIE 4576, Advanced Environmental Sensing Technology II, (22 February 2002); doi: 10.1117/12.456951
Show Author Affiliations
Israel Biran, Tufts Univ. (United States)
David M. Rissin, Tufts Univ. (United States)
David R. Walt, Tufts Univ. (United States)

Published in SPIE Proceedings Vol. 4576:
Advanced Environmental Sensing Technology II
Tuan Vo-Dinh; Stephanus Buettgenbach, Editor(s)

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