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Proceedings Paper

Multichannel optical biosensors for label-free high-throughput screening
Author(s): Petr I. Nikitin; Boris Goshkov; Mikhail V. Valeiko; Sergei Nikitin
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Paper Abstract

An optical method has been proposed and successfully tested for direct detection of biochemical reactions on a surface, which is insensitive to variations of the radiation intensity and refractive index of a solution. The method is based on detection of the spectrum of the reflected or transmitted radiation modulated by the interference in a sensitive layer of large thickness (several tens and hundreds of microns), which can be a microscope cover glass with a deposited receptor layer. A change in the phase of the interference pattern in this spectrum is used as an information signal about a change in the thickness of the sensitive layer caused by a biochemical reaction. Single- and multichannel (up to 96 channels) devices have been designed to study reactions of binding and detachment of proteins in real time. The root-mean-square noise of the prototypes expressed in the layer thickness was 3 pm.

Paper Details

Date Published: 14 February 2002
PDF: 10 pages
Proc. SPIE 4578, Fiber Optic Sensor Technology and Applications 2001, (14 February 2002); doi: 10.1117/12.456063
Show Author Affiliations
Petr I. Nikitin, Institute of General Physics (Russia)
Boris Goshkov, Institute of General Physics (Russia)
Mikhail V. Valeiko, Institute of General Physics (Russia)
Sergei Nikitin, Institute of General Physics (Russia)

Published in SPIE Proceedings Vol. 4578:
Fiber Optic Sensor Technology and Applications 2001
Michael A. Marcus; Brian Culshaw, Editor(s)

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