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Proceedings Paper

UV Raman detection of micro-organisms and their toxins in fish tissue
Author(s): Wilfred H. Nelson; Jay F. Sperry; Paul Hargraves; Eugene G. Hanlon; Ramachandra R. Dasari; Michael S. Feld
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Paper Abstract

It has been determined that domoic acid (DA) can be quantitated from homogenized shellfish tissue by means of resonance Raman spectra excited by 251 nm light. Detection limits have been found to be substantially below the 20 micrograms DA per grain tissue deemed by regulators to be unfit for human consumption. Clam tissue obtained from a supermarket has been prepared for analysis by direct homogenization for 2 minutes in a Waring blender. The homogenized samples were placed in a flow system and subjected to a 5-10 mw 251 nm excitation. Back-scattering collected for 20-30 seconds provided sufficient information for analysis. The method is extremely simple to use since the DA produces a single intense peak at 1652 cm-1. Because relatively-weak protein, nucleic acid and lipid spectra are excited from the tissue, background interference is surprisingly low. Bacteria can be detected using the same approach, but sensitivities are much lower primarily due to spectral interference from tissue nucleic acids.

Paper Details

Date Published: 22 February 2002
PDF: 12 pages
Proc. SPIE 4577, Vibrational Spectroscopy-based Sensor Systems, (22 February 2002); doi: 10.1117/12.455737
Show Author Affiliations
Wilfred H. Nelson, Univ. of Rhode Island (United States)
Jay F. Sperry, Univ. of Rhode Island (United States)
Paul Hargraves, Univ. of Rhode Island (United States)
Eugene G. Hanlon, Massachusetts Institute of Technology (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 4577:
Vibrational Spectroscopy-based Sensor Systems
Steven D. Christesen; Arthur J. Sedlacek III, Editor(s)

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