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Proceedings Paper

Quantitative phase contrast tomography using coherent synchrotron radiation
Author(s): Peter Cloetens; Wolfgang Ludwig; Elodie Boller; Lukas Helfen; Luc Salvo; Regis Mache; Michel Schlenker
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Paper Abstract

The coherence of third generation synchrotron beams makes a trivial form of phase-contrast imaging possible. It is based on propagation and corresponds to the defocusing technique of electron microscopy. The propagation technique can be used either in a qualitative way, mainly useful for edge- detection, or in a quantitative way, involving numerical retrieval of the phase from images recorded at different distances (typically three or four) from the sample. The combination with tomography allows to reconstruct the electron density in the sample with micrometer resolution. This combined approach is called holotomography. It was applied to several problems in materials and life sciences when it is crucial to enhance the sensitivity or reduce the dose compared to absorption tomography. Pure phase objects such as foams and fleece structures can be imaged with excellent contrast and resolution. Holotomography turned out to be a invaluable tool to study semi-solid materials with two metallurgical phases that have similar attenuation coefficients. The attenuation and density map yield in this case complementary information, the latter being the useful one to study the connectivity of the solid phase. The dose reduction and increased sensitivity in phase imaging are crucial for imaging thick (millimeter range) biological samples in their natural, wet environment. Results obtained on Arabidopsis plant indicate the possibility to investigate at the micron scale the spatial organisation of plant cells.

Paper Details

Date Published: 7 January 2002
PDF: 10 pages
Proc. SPIE 4503, Developments in X-Ray Tomography III, (7 January 2002); doi: 10.1117/12.452867
Show Author Affiliations
Peter Cloetens, European Synchrotron Radiation Facility (France)
Wolfgang Ludwig, European Synchrotron Radiation Facility (France)
Elodie Boller, European Synchrotron Radiation Facility (France)
Lukas Helfen, European Synchrotron Radiation Faciliyt and Fraunhofer Institute for Non-Destructive Test (Germany)
Luc Salvo, Ecole Nationale Superieure de Physique de Grenoble (France)
Regis Mache, Lab. Plastes et Differenciation Cellulaire (France)
Michel Schlenker, CNRS (France)

Published in SPIE Proceedings Vol. 4503:
Developments in X-Ray Tomography III
Ulrich Bonse, Editor(s)

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