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Proceedings Paper

System for holographic interferometry based on bacteriorhodopsin films
Author(s): Norbert A. Hampp; Thorsten Juchem
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Paper Abstract

Films made from genetically modified bacteriorhodopsins (BR) are attractive media for holographic recording. A holographic camera system is presented which uses BR-films as photowritable-photoerasable optical recording media. This camera system is useful in applications like non-destructive testing, vibration analysis and size measurement. To our knowledge this is the first technical system were a biological material, the BR-films, are employed as a performance determining component. The camera head is dust proof sealed and internally damped for applications in a technical environment. All control and display devices are mounted in a separate 19' rack which comes with the system. The system can be easily switched between various holographic measurement techniques under software control, among them double-exposure, time-averaging, real-time and phase-shifting interferometry. The system is able to resolve deformations with a resolution down to 5 nm ((lambda) /100) and operates at 12 frames/second. A variable read-out magnification allows to take advantage of the very high resolution of the BR-films which is beyond 5000 lines/mm. Polarization recording in BR-films is employed to obtain a high signal-to-noise ratio even at low exposures of 100 (mu) J/cm2. The recording mechanism at low light levels is discussed in detail.

Paper Details

Date Published: 25 October 2001
PDF: 9 pages
Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); doi: 10.1117/12.446653
Show Author Affiliations
Norbert A. Hampp, Univ. of Marburg (Germany)
Thorsten Juchem, Univ. of Marburg (Germany)

Published in SPIE Proceedings Vol. 4597:
Biophotonics Instrumentation and Analysis
Arthur E. T. Chiou; Halina Podbielska M.D.; Steven L. Jacques, Editor(s)

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