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Proceedings Paper

Autofluorescence diagnostic algorithm for detecting malignant colonic tissues
Author(s): Zhiwei Huang; Wei Zheng; Teck-Chee Chia; Cheong Hoong Diong; Tuan-Kay Lim; Francis Choen Seow; Haishan Zeng; Calum E. MacAulay; D. I. Maclean; Harvey Lui M.D.
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Paper Abstract

The ratio of autofluorescence intensity at 550+/- 10 nm to that at 630+/- 10 nm (R1=I550/I630) or 680+/- 10 nm (R2=I550/I680) was used as a diagnostic algorithm for identification of malignant tumour tissues. The performance of the ratio diagnostic algorithm was evaluated on more than 100 human colonic specimens under excitation laser light at 457.9 nm. A significant difference of the ratio value R1 and R2 was found between normal and tumour specimens (p<0.001). The diagnostic test has a sensitivity and specificity of 85% and 87% for a threshold value of R1=1.75, and a sensitivity and specificity of 94% and 82% for a threshold of R2=3.5. The experimental results show that the ratio mapping is sensitive to the small changes in the presence of tumour tissues, and the contrast of the contour map for detecting the region of malignancy can also be enhanced significantly if using the diagnostic algorithm R2 (I550/I680).

Paper Details

Date Published: 25 October 2001
PDF: 5 pages
Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); doi: 10.1117/12.446650
Show Author Affiliations
Zhiwei Huang, British Columbia Cancer Research Ctr. and Nanyang Technological Univ. (Canada)
Wei Zheng, National Cancer Ctr. (Singapore)
Teck-Chee Chia, Nanyang Technological Univ. (Singapore)
Cheong Hoong Diong, Nanyang Technological Univ. (Singapore)
Tuan-Kay Lim, Nanyang Technological Univ. (Singapore)
Francis Choen Seow, Singapore General Hospital (Singapore)
Haishan Zeng, British Columbia Cancer Research Ctr. (Canada)
Calum E. MacAulay, British Columbia Cancer Research Ctr. (Canada)
D. I. Maclean, Univ. of British Columbia (Canada)
Harvey Lui M.D., Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 4597:
Biophotonics Instrumentation and Analysis
Arthur E. T. Chiou; Halina Podbielska M.D.; Steven L. Jacques, Editor(s)

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