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Proceedings Paper

Using a laser-Doppler flowmetry to measure pulsatile microcirculation on the kidney in rats
Author(s): Ming-Yie Jan; Pin-Tsun Chao; Tse-Lin Hsu; Yuh-Yin Lin Wang; Wei-Kung Wang
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Paper Abstract

Although Laser Doppler flowmetery (LDF) been extensively used in measurement of microvascular blood flow of different tissues. However, due to some physiological vibrations, fast oscillations of the renal cortical flux (RCF) are hard to be measured. In the study, a commercial 3mW 780nm Laser Doppler flowmetery, with a single fiber and a de-vibration holder, was used to measure the pulsatile RCF in rats. Considering the fast response due to the heart rate of rats, the time constant (TC) was set to 0.05 second and thus the frequency response is up to 20Hz. Furthermore, a calibration standard and a static blood sample were also measured as the references without the pulsatile driving force. In order not to perturb the RCF with tiny momentum, the applying force that the fiber exerted on the renal surface was controlled below 100 dyne. To enhance the signal to noise ratio (SNR), an averaged periodogram was used to estimate the frequency components of the pulsatile microcirculation. It is found that the dominating fast oscillation of RCF is pulsatile and its harmonic components are directly correlated with those of the heartbeat (correlation coefficient =0.999, P<0.001, n=17). The result shows that, in the kidney, the pulsatile RCF is the dominating component of microcirculation oscillation and driven by the fast propagating blood pressure. This technique could be further utilized to analyze the pharmacological effect and hemodynamic parameters on renal function.

Paper Details

Date Published: 25 October 2001
PDF: 6 pages
Proc. SPIE 4597, Biophotonics Instrumentation and Analysis, (25 October 2001); doi: 10.1117/12.446636
Show Author Affiliations
Ming-Yie Jan, Institute of Physics (Taiwan)
Pin-Tsun Chao, Institute of Physics and National Taiwan Univ. (Taiwan)
Tse-Lin Hsu, Institute of Physics (Taiwan)
Yuh-Yin Lin Wang, Institute of Physics and National Taiwan Normal Univ. (Taiwan)
Wei-Kung Wang, Institute of Physics and National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 4597:
Biophotonics Instrumentation and Analysis
Arthur E. T. Chiou; Halina Podbielska M.D.; Steven L. Jacques, Editor(s)

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