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Proceedings Paper

Myocardial expression of the vascular endothelial growth factor (VEGF) after endocardial laser revascularization (ELR)
Author(s): Jan Rommerscheid; Dirk Theisen; G. Schmuecker; Ralf Brinkmann; R. Broll
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Paper Abstract

Background. Endocardial laser revascularization (ELR) is a new technique to treat patients with severe coronary artery disease (CAD) in a percutaneous approach. The results show a significant improvement of symptoms, but the mechanism of action is still unknown. One main theory is the angiogenesis for which Vascular Endothelial Growth Factor (VEGF) is the keypromotor. We investigated immunohistochemically the VEGF-expression after ELR in porcine hearts over a timeperiod of four weeks. Methods. ELR was performed with a single-pulse Thulium:YAG laser. 15 pigs were treated with ELR and the hearts were harvested at five timeperiods: directly (group I), 3 days (group II), 1 week (group III), 2 weeks (group IV) and 4 weeks (group V) after ELR. Each group consisted of three pigs. Immunohistochemically the VEGF-expression was assessed by staining with a polyclonal antibody against VEGF and cellcounting using an expression index (VEGF-EI) Results. A maximum of VEGF-expression was found three days (group II) after ELR with a VEGF-EI of 97%. At 1 week (group III) the VEGF-EI was similar high with 93%. Along the timecourse the index decreased to 22% at 4 weeks (groupV). Conclusions. Our findings show that ELR leads to an local upregulation of VEGF around the channels. The resulting angiogenesis could be the mechanism for the relief of angina.

Paper Details

Date Published: 26 October 2001
PDF: 6 pages
Proc. SPIE 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy, (26 October 2001); doi: 10.1117/12.446529
Show Author Affiliations
Jan Rommerscheid, Univ. Clinic Luebeck (Germany)
Dirk Theisen, Medical Laser Ctr. Luebeck GmbH (Germany)
G. Schmuecker, Medical Laser Ctr. Luebeck GmbH (Germany)
Ralf Brinkmann, Medical Laser Ctr. Luebeck GmbH (Germany)
R. Broll, Univ. Clinic Luebeck (Germany)

Published in SPIE Proceedings Vol. 4433:
Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy
Reginald Birngruber; Hubert van den Bergh, Editor(s)

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