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Proceedings Paper

Antidiabetics and diuretics show phototoxicity in HaCaT cells
Author(s): Edgar Selvaag M.D.; Anita B. Petersen; Robert Gniadecki; Tine Thorn; Hans Christian Wulf
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Paper Abstract

The antidiabetics tolbutamide, glibenclamide, and glipizide, and the diuretics bendroflumethiazide, butizide, furosemide, hydrochlorothiazide, and trichlormethiazide were investigated for potential phototoxicity in the HaCaT cell line. The cells were incubated with the drugs and then exposed to UVA1 irradiation. The effects of the antioxidants L-ascorbic acid, and (alpha) -tocopherol on oxidative DNA damage were assessed. Bendroflumethiazide, furosemide, hydrochlorothiazide, trichlormethiazide, or tolbutamide induced dose-dependent phototoxicity. Cells incubated with bendroflumethiazide, tolbutamide, and glibenclamide, and irradiated with UVA1 demonstrated an increased oxidative DNA damage. Pre-treatment with L-ascorbic acid, or (alpha) -tocopherol, suppressed the UVA-induced DNA damage in cells incubated with 1 mM of bendroflumethiazide, furosemide, glibenclamide, glipizide, tolbutamide, and trichloromethiazide, further implying the involvement of reactive oxygen species in the phototoxic DNA damage. These results may indicate a link between phototoxic and photocancerogenic potential of the sulfonamide-derived oral antidiabetic and diuretic drugs, as it has previously been recognized for psoralen, chlorpromazine, and fluoroquinolones. Excessive exposure to UV light may be deleterious for patients treated with these drugs.

Paper Details

Date Published: 26 October 2001
PDF: 2 pages
Proc. SPIE 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy, (26 October 2001); doi: 10.1117/12.446520
Show Author Affiliations
Edgar Selvaag M.D., Bispebjerg Hospital (Germany)
Anita B. Petersen, Bispebjerg Hospital (Denmark)
Robert Gniadecki, Bispebjerg Hospital (Denmark)
Tine Thorn, Bispebjerg Hospital (Denmark)
Hans Christian Wulf, Bispebjerg Hospital (Denmark)

Published in SPIE Proceedings Vol. 4433:
Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy
Reginald Birngruber; Hubert van den Bergh, Editor(s)

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