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Proceedings Paper

Fluence rate effects in meta-tetra(hydroxyphenyl)chlorin (mTHPC) photosensitized HT29 multicell tumor spheroids: comparative study with Colo26 spheroids
Author(s): Estelle Maugain; Stephanie Coutier; Lina N. Bezdetnaya; Sophie Marchal; Jean-Louis Merlin; Jacques Didelon; Francois H. Guillemin
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Paper Abstract

The present study evaluates the impact of the fluence rate of red light irradiation ((lambda) = 650 nm) on photodynamic efficacy in mTHPC-sensitized HT29 and Colo26 spheroids. Photocytotoxicity, computed as product of the cell yield and plating efficiency, increased progressively when the fluence rate was reduced from 90 to 30 mW cm-2 in both types of spheroids. The observed photocytotoxicity was mainly due to an immediate rather than a delayed cell death for HT29 spheroids. An opposite effect was observed for Colo26 spheroids. The plausible explanations could be the difference in penetration of mTHPC into the spheroids as well as their heterogeneous structure.

Paper Details

Date Published: 26 October 2001
PDF: 7 pages
Proc. SPIE 4433, Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy, (26 October 2001); doi: 10.1117/12.446517
Show Author Affiliations
Estelle Maugain, Ctr. Alexis Vautrin (France)
Stephanie Coutier, Ctr. Alexis Vautrin (France)
Lina N. Bezdetnaya, Ctr. Alexis Vautrin (France)
Sophie Marchal, Ctr. Alexis Vautrin (France)
Jean-Louis Merlin, Ctr. Alexis Vautrin (France)
Jacques Didelon, Ctr. Alexis Vautrin (France)
Francois H. Guillemin, Ctr. Alexis Vautrin (France)

Published in SPIE Proceedings Vol. 4433:
Laser-Tissue Interactions, Therapeutic Applications, and Photodynamic Therapy
Reginald Birngruber; Hubert van den Bergh, Editor(s)

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