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Proceedings Paper

Peptide nucleic acid (PNA) biosensors for DNA recognition
Author(s): Jiong Li; Hong Wang; Lu Cheng; Yujie Zhao; Heping Liu; Nongyao He; Zuhong Lu
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Paper Abstract

PNA has demonstrated remarkable hybridization properties towards complementary oligonucleotides. In this paper, we tested the PNA's ability of selectively discriminating against single-base mismatches. Efforts were paid to optimizing the fabrication of PNA biosensors and its hybridization to achieve better discriminating capacity. Different factors were studied such as immobilization concentration, target concentration, ion strength and hybridization temperature. An optimized procedure was produced to reliably detect DNAs with single-base mismatches in the sequences. We synthesized two 16 mer PNAs with single-base mismatch to hybridizing with complementary DNA. The complementary PNA showed 8.6 fold stronger signal intensity than mismatch one under our optimized procedure.

Paper Details

Date Published: 15 October 2001
PDF: 5 pages
Proc. SPIE 4601, Micromachining and Microfabrication Process Technology and Devices, (15 October 2001); doi: 10.1117/12.444753
Show Author Affiliations
Jiong Li, Southeast Univ. (China)
Hong Wang, Southeast Univ. (China)
Lu Cheng, Southeast Univ. (China)
Yujie Zhao, Southeast Univ. (China)
Heping Liu, Southeast Univ. (China)
Nongyao He, Southeast Univ. (China)
Zuhong Lu, Southeast Univ. (China)

Published in SPIE Proceedings Vol. 4601:
Micromachining and Microfabrication Process Technology and Devices
Norman C. Tien; Qing-An Huang, Editor(s)

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