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Proceedings Paper

Fabrication of polyurethane molecular stamps for the synthesis of DNA microarray
Author(s): Zhengchun Liu; Quanguo He; Pengfeng Xiao; Nongyao He; Zuhong Lu; Liang Bo
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Paper Abstract

Polyurethane based on polypropylene glycol (PPG) and Toluene diisocyanate (TDI) using 3,3'-dichloride-4,4'- methylenedianiline (MOCA) as the crosslinker is presented for the first time to fabricate molecular stamps (PU stamps) for the synthesis of DNA microarray with contact procedure. The predictability of the process is achieved by utilizing commercially available starting materials. SEM analysis of the morphology of PU stamps and master showed that PU elastometer could replicate subtly the motherboard's patterns with high fidelity. It was proved from the contact angle measurement that PU stamps surface has good affinity with acetonitrile, which guarantee the well-distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays confirmed polyurethane is an excellent material for molecular stamps.

Paper Details

Date Published: 15 October 2001
PDF: 5 pages
Proc. SPIE 4601, Micromachining and Microfabrication Process Technology and Devices, (15 October 2001); doi: 10.1117/12.444752
Show Author Affiliations
Zhengchun Liu, Southeast Univ. and Huaihua Teacher's College (China)
Quanguo He, Southeast Univ. (China)
Pengfeng Xiao, Southeast Univ. (China)
Nongyao He, Southeast Univ. (China)
Zuhong Lu, Southeast Univ. (China)
Liang Bo, Xiangtan Univ. (China)

Published in SPIE Proceedings Vol. 4601:
Micromachining and Microfabrication Process Technology and Devices
Norman C. Tien; Qing-An Huang, Editor(s)

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