Share Email Print

Proceedings Paper

Manipulation of single-DNA molecules and measurements of their elastic properties under an optical microscope
Author(s): Carlos J. Bustamante; Laura Finzi; Page E. Sebring; Steven B. Smith
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Single molecules of DNA can be visualized in solution by epifluorescence microscopy, manipulated and extended by a variety of mechanical, electrical and chemical means as described previously. This has been used to design experiments under an optical microscope, in which DNA molecules are extended by a known force, to determine the elastic response of the molecules, both in the presence and absence of ethidium bromide. It is found that at lower forces (smaller extensions) the molecules behave as entropic springs with a persistence length of 500 angstroms, and that at the ionic strengths used, the intercalation of ethidium bromide does not alter this persistence length, while it appears to elongate the contour length of the molecule by about 30%.

Paper Details

Date Published: 1 July 1991
PDF: 9 pages
Proc. SPIE 1435, Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications, (1 July 1991); doi: 10.1117/12.44242
Show Author Affiliations
Carlos J. Bustamante, Univ. of Oregon (United States)
Laura Finzi, Univ. of Oregon (United States)
Page E. Sebring, Univ. of Oregon (United States)
Steven B. Smith, Univ. of Oregon (United States)

Published in SPIE Proceedings Vol. 1435:
Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications
Bryan L. Fearey, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?