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Proceedings Paper

Single-color laser-induced fluorescence detection and capillary gel electrophoresis for DNA sequencing
Author(s): Da Yong Chen; Harold Swerdlow; Heather Harke; Jian Zhong Zhang; Norman J. Dovichi
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Paper Abstract

Low zeptomole (1 zmol equals 10-21 mol = 600 molecules) detection limits are produced for DNA sequencing by capillary gel electrophoresis. A 750 (mu) w green helium-neon laser ((lambda) equals 543.5 nm) is used to excite tetramethylrhodamine-labeled DNA fragments in a sheath-flow cuvette. A cooled photomultiplier tube is used to detect fluorescence in a single spectral channel. Sequencing data is generated at a rate of about 70 bases/hour.

Paper Details

Date Published: 1 July 1991
PDF: 7 pages
Proc. SPIE 1435, Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications, (1 July 1991); doi: 10.1117/12.44240
Show Author Affiliations
Da Yong Chen, Univ. of Alberta (Canada)
Harold Swerdlow, Univ. of Alberta (United States)
Heather Harke, Univ. of Alberta (Canada)
Jian Zhong Zhang, Univ. of Alberta (Canada)
Norman J. Dovichi, Univ. of Alberta (United States)

Published in SPIE Proceedings Vol. 1435:
Optical Methods for Ultrasensitive Detection and Analysis: Techniques and Applications
Bryan L. Fearey, Editor(s)

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