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Proceedings Paper

Direct measurement of vibrational energy relaxation in photoexcited deoxyhemoglobin using picosecond Raman spectroscopy
Author(s): John B. Hopkins; Xiaobing Xu; Robert Lingle Jr.; Huiping Zhu; Soo-chang Yu
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Paper Abstract

We have directly observed hot vibrations in photoexcited deoxyhemoglobin. The data quantitatively indicate a heme vibrational temperature of 20 K above room temperature within the time sampled by an 8ps laser pulse. The slow component of vibrational relaxation occurs with a rate constant of (2.5ps)1. Similar dynamics are observed in oxyhemoglobin. The initial stages of recombmation of 02 with the heme following photodissociation of oxyhemoglobin are observed in the transient vibrational spectrum. Contrary to previous work, we do not observe the influence of protein dynamics on 02 recombination.

Paper Details

Date Published: 1 June 1991
PDF: 6 pages
Proc. SPIE 1432, Biomolecular Spectroscopy II, (1 June 1991); doi: 10.1117/12.44224
Show Author Affiliations
John B. Hopkins, Louisiana State Univ. (United States)
Xiaobing Xu, Louisiana State Univ. (United States)
Robert Lingle Jr., Louisiana State Univ. (United States)
Huiping Zhu, Louisiana State Univ. (United States)
Soo-chang Yu, Louisiana State Univ. (United States)

Published in SPIE Proceedings Vol. 1432:
Biomolecular Spectroscopy II
Robert R. Birge; Laurence A. Nafie, Editor(s)

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