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Proceedings Paper

Detection and localization of absorbers in scattering media using frequency-domain principles
Author(s): Klaus W. Berndt; Joseph R. Lakowicz
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Paper Abstract

We report on time-resolved reflectance imaging experiments on a scattering medium containing a spatially limited absorber. The medium is illuminated at two positions with pulses from a mode-locked and cavity dumped picosecond dye laser. Time-resolved imaging of the back-scattered light is realized by means of a RF-phase-sensitive camera, synchronized to the laser pulses. The camera consists of a sinusoidally modulated proximity-focused image intensifier, a thermo-electrically cooled CCD camera, and a digital image processor unit. In operation, at least two images are taken under different image intensifier modulation conditions, such as modulation phase or modulation degree. By processing the stored images, a final image can be created the contrast of which is based only on time differences of the back-scattered photons. We found that this image reveals the presence and, to a certain extent, the position of a spatially limited absorber within the scattering medium. These experiments have been performed to evaluate possible ways towards an eventual optical tomography in living tissue.

Paper Details

Date Published: 1 May 1991
PDF: 12 pages
Proc. SPIE 1431, Time-Resolved Spectroscopy and Imaging of Tissues, (1 May 1991); doi: 10.1117/12.44186
Show Author Affiliations
Klaus W. Berndt, Univ. of Maryland School of Medicine (United States)
Joseph R. Lakowicz, Univ. of Maryland School of Medicine (United States)

Published in SPIE Proceedings Vol. 1431:
Time-Resolved Spectroscopy and Imaging of Tissues
Britton Chance, Editor(s)

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