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Proceedings Paper

Fluorescence characteristics of atherosclerotic plaque and malignant tumors
Author(s): Stefan Andersson-Engels; Luc Baert M.D.; Roger Berg; Marie-Ange D'Hallewin M.D.; Jonas Johansson; Unne Stenram; Katarina Svanberg M.D.; Sune Svanberg
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Paper Abstract

Two series of investigations utilizing laser-induced fluorescence (LIF) in characterizing diseased tissue are presented. In one in vitro investigation the fluorescence from normal and atherosclerotically diseased arteries are studied. In another clinical study the fluorescence in vivo from superficial urinary bladder malignancies in patients who had received a low-dose injection of Hematoporphyrin Derivative (HpD) is investigated. Additionally, the fluorescence properties of L-tryptophan, collagen-I powder, elastin powder, nicotinamide adenine dinucleotide and (beta) -carothene were investigated and compared with the spectra from the tissue samples. A nitrogen laser (337 nm) alone or in connection with a dye laser (405 nm) was used together with an optical multichannel analyzer (OMA) to study the fluorescence spectra. The fluorescence decay characteristics of atherosclerotic plaque were examined utilizing a mode locked argon ion laser, synchronously pumping a picosecond dye laser. A fast detection system based on photon counting was employed. The fluorescence decay curves were evaluated on a PC computer allowing up to three lifetime components to be determined. A fluorescence peak at 390 nm in fibrotic plaque was identified as due to collagen fibers, while a fluorescence peak at 520 nm was connected to (beta) -carotene. The in vivo measurements of urinary bladder malignancies were performed with the optical fiber of the OMA system inserted through the biopsy channel of a cystoscope during the diagnostical procedure. The spectral recordings from urinary bladders, obtained at 337 nm and 405 nm excitation, revealed fluorescence features which can be used to demarcate tumor areas from normal mucosa. The fluorescence emission might also be useful to characterize different degrees of dysplasia.

Paper Details

Date Published: 1 June 1991
PDF: 13 pages
Proc. SPIE 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, (1 June 1991); doi: 10.1117/12.44046
Show Author Affiliations
Stefan Andersson-Engels, Lund Institute of Technology (Sweden)
Luc Baert M.D., Univ. Hospital St. Pieter (Belgium)
Roger Berg, Lund Institute of Technology (Sweden)
Marie-Ange D'Hallewin M.D., Univ. Hospital St. Pieter (Belgium)
Jonas Johansson, Lund Institute of Technology (Sweden)
Unne Stenram, Lund Univ. Hospital (Sweden)
Katarina Svanberg M.D., Lund Univ. Hospital (Sweden)
Sune Svanberg, Lund Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 1426:
Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques
Thomas J. Dougherty, Editor(s)

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