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Proceedings Paper

Optical DNA biosensor based on molecular beacon immobilized on sol-gel membrane
Author(s): Jun Li; Weihong Tan; Kemin Wang; Xiaohai Yang; Zhiwen Tang; Xiaoxiao He
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Paper Abstract

Novel biotinylated molecular beacon (MB) probe was developed to prepare DNA biosensor using sol-gel chemistry. Biotinylated bovine serum albumin was encapsulated in the sol-gel matrix to form a biotinylated silica gel. Streptavidin acted as a bridge between biotinylated MB and silica gel. The MB based DNA biosensor can detect nonlabeled DNA target in real time with high sensitivity and one-base- mismatch selectivity, which indicated the potential applications in single point mutation detection. Compared with the previous report, the sol-gel based DNA biosensor has three advantages: (1). The response time was significantly shortened to 10 minutes when the cDNA concentration was over 10 nM. (2). The silica gel prolonged the lifetime of the MB based biosensor, which could keep its activity for thee weeks if stored in a buffer. (3) The gel formed by sol-gel method has a larger surface area than the common glass cover at the same scope. The number of binding sites for biotinylated increased significantly. Hence the detection limit was down to subnanomolar/L.

Paper Details

Date Published: 14 September 2001
PDF: 4 pages
Proc. SPIE 4414, International Conference on Sensor Technology (ISTC 2001), (14 September 2001); doi: 10.1117/12.440191
Show Author Affiliations
Jun Li, Hunan Univ. (China)
Weihong Tan, Hunan Univ. (China)
Kemin Wang, Hunan Univ. (China)
Xiaohai Yang, Hunan Univ. (China)
Zhiwen Tang, Hunan Univ. (China)
Xiaoxiao He, Hunan Univ. (China)

Published in SPIE Proceedings Vol. 4414:
International Conference on Sensor Technology (ISTC 2001)
Yikai Zhou; Shunqing Xu, Editor(s)

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