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Proceedings Paper

Sensitive fiber-optic immunoassay
Author(s): Irene M. Walczak; Walter F. Love; Rudolf E. Slovacek
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Paper Abstract

The principles of evanescent wave theory were applied to an immunological sensor for detecting the cardiac-specific isoenzyme creatine kinase-MB (CK-MB). The detection of the CK-MB isoenzyme is used in conjunction with the total CK measurement in the diagnosis of acute myocardial infarction. The clinical range for CK-MB is from 2-100 ng/ml. Previous work which utilized the fluorophor, Fluorescein isothiocyanate (FITC), was able to discriminate between 0 and 3 ng/ml CK-MB. Use of the fluorophor B-phycoerythrin (BPE) increased the assay sensitivity to 0.1 ng/ml CK-MB. The data was collected for 15 minutes using an optical launch and collection angle of 25 degree(s). This fiber optic based system is homogeneous and requires no subsequent washing, handling, or processing steps after exposure to the sample.

Paper Details

Date Published: 1 July 1991
PDF: 11 pages
Proc. SPIE 1420, Optical Fibers in Medicine VI, (1 July 1991); doi: 10.1117/12.43859
Show Author Affiliations
Irene M. Walczak, Ciba Corning Diagnostic Corp. (United States)
Walter F. Love, Corning Incorporated (United States)
Rudolf E. Slovacek, Ciba Corning Diagnostic Corp. (United States)

Published in SPIE Proceedings Vol. 1420:
Optical Fibers in Medicine VI
Abraham Katzir, Editor(s)

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