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Proceedings Paper

3D imaging of lung tissue by confocal microscopy and micro-CT
Author(s): Andres Kriete; Andreas Breithecker; Wigbert D. Rau
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Paper Abstract

Two complementary techniques for the imaging of tissue subunits are discussed. A computer guided light microscopic imaging technique is described first, which confocally resolves thick serial sections axially. The lateral area of interest is increased by scanning a mosaic of images in each plane. Subsequently, all images are fused digitally to form a highly resolved volume exhibiting the fine structure of complete respiratory units of lung. A different technique described is based on microtomography. This method allows to image volumes up to 3x3x3 cm at a resolution of up to 7 microns. Due to the lack of strong density differences, a contrast enhancement procedure is introduced which makes this technique applicable for the imaging of lung tissue. Imaging, visualization and analysis described here are parts of an ongoing project to model structure and to simulate function of tissue subunits and complete organs.

Paper Details

Date Published: 9 July 2001
PDF: 8 pages
Proc. SPIE 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, (9 July 2001); doi: 10.1117/12.434736
Show Author Affiliations
Andres Kriete, Univ. of Giessen (United States)
Andreas Breithecker, Univ. Clinic Giessen (Germany)
Wigbert D. Rau, Univ. Clinic Giessen (Germany)

Published in SPIE Proceedings Vol. 4257:
Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical
Donald Dean Duncan; Donald Dean Duncan; Steven L. Jacques; Peter C. Johnson; Peter C. Johnson, Editor(s)

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