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Proceedings Paper

Kinetic of antigent-antibody reactions with scattering method
Author(s): Olexander I. Bilyi; Yevgen M. Kiselyov; Volodymyr P. Novikov
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Paper Abstract

The immune reactions of interaction antigen-antibody represent specific effect of an antigene with an antibody, which outcome are the complex immune aggregates forming precipitate in case of a soluble antigene, or agglutinate in case of a corpuscular antigene. Immunological methods which uses in the quality of carrier protein latex's polymeric microspheresis, gained name and method latex agglutination. Polymeric microspheresis have the array of advantages before biological carries, which consist in the opportunity of the variation of attributes surface and size microspheresis in the broad band of meanings with the preservation of narrow distribution particles behind measurements, the putting of functional groups, necessary for bunch with ligand on stage their synthesis, in ragidity at storage. The quantitative evaluation of parameters of a response of interaction antigen-antibody in immunology is possible by optical methods on a measurement of a modification of intensity of a light stream of a solution in an outcome of a course of a reaction. Concentration of immune complexes determine both on slacking a taking place stream of light, and on a modification of intensity of a stream of light scattering suspended particles in a solution. The process light scattering by colloidal aggregates are formed from suspension microspheresis with adsorbed on their surface protein is described. In report the physics principle of registration immune reaction by light scattering methods is concerned. The results of the effectiveness latex's preparation created on basis of the polymeric carries is described.

Paper Details

Date Published: 11 July 2001
PDF: 6 pages
Proc. SPIE 4515, Light and Optics in Biomedicine, (11 July 2001); doi: 10.1117/12.432972
Show Author Affiliations
Olexander I. Bilyi, The Ivan Franko National Univ. of Lviv (Ukraine)
Yevgen M. Kiselyov, Lvivska Polytechnica State Univ. (Ukraine)
Volodymyr P. Novikov, Lvivska Polytechnica State Univ. (Ukraine)

Published in SPIE Proceedings Vol. 4515:
Light and Optics in Biomedicine
Maksymilian Pluta; Anna Cysewska-Sobusiak, Editor(s)

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