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Proceedings Paper

Compact hyperspectral imager for low-light applications
Author(s): Petr Herman; Henryk M. Malak; Wayne E. Moore; Yaroslav Vecer
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Paper Abstract

A high-performance hyperspectral imaging module with high light throughput suitable for microscopy and analytical imaging was built and tested. The imager utilizes the phenomenon of optical activity. The new technique provides information from a continuous spectral range of 250 - 1000nm. Similar spectral range extended to the near IR is also achievable. The imager has the form of a small module which can be inserted between a microscope or other imaging system and a camera. We have tested an 8-bit CCD video-rate camera with satisfactory results. The resulting instrument is simple, robust, and highly compact. The imager module is placed in-line to the microscope imaging system and does not introduce observable image aberrations. The imager is transparent to conventional imaging operations, thus with the imager in-place there is no need for reconfiguration of the microscope or switching between conventional and hyperspectral video/digital imaging modes. The presented spectral imager answers the need for a sensitive, compact, and affordable imaging spectrometer. The instrument is suited for applications requiring parallel acquisition of highly resolved concurrent spatial and spectral information such as high throughput screening, biochip analysis, remote sensing, semiconductor testing, etc. Images, spectral maps, and spectra of various fluorescent objects are presented.

Paper Details

Date Published: 2 July 2001
PDF: 8 pages
Proc. SPIE 4259, Biomarkers and Biological Spectral Imaging, (2 July 2001);
Show Author Affiliations
Petr Herman, Univ. of Maryland/Baltimore and Charles Univ. (United States)
Henryk M. Malak, Microcosm, Inc. (United States)
Wayne E. Moore, Microcosm, Inc. (United States)
Yaroslav Vecer, Charles Univ. (Czech Republic)

Published in SPIE Proceedings Vol. 4259:
Biomarkers and Biological Spectral Imaging
Gregory H. Bearman; Darryl J. Bornhop; Darryl J. Bornhop; Richard M. Levenson, Editor(s)

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