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Proceedings Paper

Spectral karyotyping (SKY) in hematological neoplasia
Author(s): Birgitte S. Preiss; Rikke K. Pedersen; Gitte B. Kerndrup M.D.
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Paper Abstract

From November 1, 1997 till November 1, 2000 we have investigated 204 cases of acute myeloid leukemia (AML) (nequals95), acute lymphatic leukemia (ALL) (nequals40), myelodysplastic syndrome (MDS) (nequals11), chronic myeloid leukemia (CML) (nequals9), chronic lymphatic leukemia (CLL) (nequals4) and non-Hodgkin lymphoma (NHL) (nequals45) cytogenetically, using G-band analysis and spectral karyotyping (SKY). By SKY we were able to detect the abnormal clones in all cases but 9. In the G-band preparations these cases showed very few abnormal mitoses. The SKY either extended or confirmed the G-band findings in 94% of those with an abnormal karyotype. Cryptic translocations (translocations not suspected from the G-band karyotype) were found in 71 cases (26 AML, 9 ALL, 5 MDS, 2 CLL and 29 NHL). We find SKY a powerful adjuvant diagnostic tool that does not compromise one of the advantages of karyotyping techniques, the analysis of the entire genome which, in contrast to molecular biological techniques, still leave the possibility to get mroe answers than questions posed.

Paper Details

Date Published: 2 July 2001
PDF: 4 pages
Proc. SPIE 4259, Biomarkers and Biological Spectral Imaging, (2 July 2001); doi: 10.1117/12.432480
Show Author Affiliations
Birgitte S. Preiss, Odense Univ. Hospital (Denmark)
Rikke K. Pedersen, Odense Univ. Hospital (Denmark)
Gitte B. Kerndrup M.D., Odense Univ. Hospital (Denmark)

Published in SPIE Proceedings Vol. 4259:
Biomarkers and Biological Spectral Imaging
Gregory H. Bearman; Darryl J. Bornhop; Darryl J. Bornhop; Richard M. Levenson, Editor(s)

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