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Proceedings Paper

Bioconjugates of luminescent CdSe-ZnS quantum dots with an engineered two-domain protein G for use in fluoroimmunoassays
Author(s): Phan T. Tran; Ellen R. Goldman; Hedi M. Mattoussi; George P. Anderson; J. Matthew Mauro
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Paper Abstract

Colloidal semiconductor quantum dots (QDs) seem suitable for labeling certain biomolecules for use in fluorescent tagging applications, such as fluoro-immunoassays. Compared to organic dye labels, Qds are resistant to photo-degradation, and these luminescent nanoparticles have size-dependent emission spectra spanning a wide range of wavelengths in the visible and near IR. We previously described an electrostatic self-assembly approach for conjugating highly luminescent colloidal CdSe-ZnS core-shell Qds with engineered two-domain recombinant proteins. Here we describe the application of this approach to prepare QD conjugates with the (Beta) 2 immunoglobin G (IgG) binding domain of streptococcal protein G (PG) appended with a basic lucine zipper attachment domain (PG-zb). We also demonstrate that the QD/PG conjugates retain their ability to bind IgG antibodies, and that a specific antibody coupled to QD via the PG functional domain efficiently binds its antigen. These preliminary results indicate that electrostatically self-assembled QD/PG-zb/IgG bioconjugates can be used in fluoro-immunoassays.

Paper Details

Date Published: 18 June 2001
PDF: 7 pages
Proc. SPIE 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications, (18 June 2001); doi: 10.1117/12.430766
Show Author Affiliations
Phan T. Tran, Naval Research Lab. (United States)
Ellen R. Goldman, George Mason Univ. (United States)
Hedi M. Mattoussi, Naval Research Lab. (United States)
George P. Anderson, Naval Research Lab. (United States)
J. Matthew Mauro, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 4258:
Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications
Catherine J. Murphy, Editor(s)

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