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Proceedings Paper

Nanoparticle-amplified surface plasmon resonance for detection of DNA hybridization
Author(s): Glenn P. Goodrich; Sheila R. Nicewarner; Lin He; Michael J. Natan; Christine D. Keating
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Paper Abstract

In recent years there has been a great deal of interest in the measurement of DNA hybridization at surfaces. Surface-confined DNA hybridization has been used to monitor gene expression, to detect the presence of a particular DNA sequence and determine single nucleotide polymorphisms (SNPs). DNA microarrays, which can contain thousands of discrete DNA sequences on a single surface, have become widely used for hybridization studies. While a powerful technique, this technology is limited by the stability of the fluorescent dyes used to label the DNA, and the need to perform measurements ex-situ to reduce the fluorescence background. In this report, we describe the use of colloid-amplified surface plasmon resonance (SPR) to measure DNA hybridization at surfaces. SPR is a surface sensitive technique, which can be used to study hybridization in situ, and the use of colloidal metal tags provides excellent sensitivity. Angle-scanning SPR has been used to study oligonucleotide hybridization to surface confined probes, and work is underway to apply SPR imaging to study DNA hybridization in macro- and microarray formats.

Paper Details

Date Published: 18 June 2001
PDF: 6 pages
Proc. SPIE 4258, Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications, (18 June 2001); doi: 10.1117/12.430764
Show Author Affiliations
Glenn P. Goodrich, The Pennsylvania State Univ. (United States)
Sheila R. Nicewarner, The Pennsylvania State Univ. (United States)
Lin He, SurroMed, Inc. (United States)
Michael J. Natan, SurroMed, Inc. (United States)
Christine D. Keating, The Pennsylvania State Univ. (United States)

Published in SPIE Proceedings Vol. 4258:
Nanoparticles and Nanostructured Surfaces: Novel Reporters with Biological Applications
Catherine J. Murphy, Editor(s)

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