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Proceedings Paper

Adaptive functional support for bioreceptors based on protein layers
Author(s): K. V. Kostyukevych; Evgenie F. Venger; E. V. Rengevich; Sergey A. Kostyukevych; S. V. Verevka; P. M. Boltovets; N. S. Dyachenko
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Paper Abstract

The formation of AuxSy interfacial layer by reactive annealing of gold films in H2S atmosphere is investigated. This seems to be a technologically favorable technique for the large-scale and low-cost fabrication of nondestructive immobilization support for biological molecules. Formation of phases with different chemical functionality and surface topography as a function of reaction time was studied using Atomic Force Microscopy (AFM), Surface Plasmon Resonance (SPR) measurements and biomolecular interaction analysis [trypsin -- Soybean Trypsin Inhibitor (STI) reaction]. The results obtained confirm the classical two-step model for the sulfide phase formation during reactive annealing. This includes an intermediate formation of a dispersed phase of sulfur followed by its reconstruction to a close-packed sulfide layer. Adsorption of proteins onto a certain sulfide layer passes with retention of a native state of adsorbed molecules. The proposed strategy for formation of biochemical structures (gold/sulfide/proteins) on the surface of physical transducers open a new way for design and development of novel artificial smart sensing systems. They not only maintain optimal functioning of bioreceptors but also are responsive to their environment.

Paper Details

Date Published: 12 June 2001
PDF: 12 pages
Proc. SPIE 4425, Selected Papers from the International Conference on Optoelectronic Information Technologies, (12 June 2001); doi: 10.1117/12.429723
Show Author Affiliations
K. V. Kostyukevych, Institute of Semiconductor Physics (Ukraine)
Evgenie F. Venger, Institute of Semiconductor Physics (Ukraine)
E. V. Rengevich, Institute of Semiconductor Physics (Ukraine)
Sergey A. Kostyukevych, Institute of Semiconductor Physics (Ukraine)
S. V. Verevka, Palladin Institute of Biochemistry (Ukraine)
P. M. Boltovets, Institute of Microbiology and Virology (Ukraine)
N. S. Dyachenko, Institute of Microbiology and Virology (Ukraine)

Published in SPIE Proceedings Vol. 4425:
Selected Papers from the International Conference on Optoelectronic Information Technologies
Sergey V. Svechnikov; Volodymyr P. Kojemiako; Sergey A. Kostyukevych, Editor(s)

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