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Proceedings Paper

Online visualization of axial intensity distribution in spatially modulated illumination microscopy
Author(s): Benno Albrecht; Rainer Heintzmann; Christoph G. Cremer
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Paper Abstract

Recently, several light microscopical approaches, such as point spread function (PSF) engineering, Spectral Precision Distance Microscopy (SPDM) and related methods have demonstrated the feasibility of measurements considerably beyond the conventional Abbe-Limit of optical resolution in far field light microscopy. The Heidelberg Spatially Modulated Illumination (SMI) microscope is based on the generation of a standing wave light field in the direction of the optical axis of the system. The theoretical PSF is obtained by the manipulation of the sin2-shaped illumination PSF and the epifluorescent detection PSF. Here, we report a software method to obtain online visualization of the axial light distribution of any object detected, developed using Microsoft Visual C++ and based on Windows NT. This strongly facilitates routine application of SMI Microscopy.

Paper Details

Date Published: 10 May 2001
PDF: 5 pages
Proc. SPIE 4260, Optical Diagnostics of Living Cells IV, (10 May 2001); doi: 10.1117/12.426781
Show Author Affiliations
Benno Albrecht, Univ. of Heidelberg (Germany)
Rainer Heintzmann, Max-Planck Institute for Biophysical Chemistry (Germany)
Christoph G. Cremer, Univ. of Heidelberg (Germany)

Published in SPIE Proceedings Vol. 4260:
Optical Diagnostics of Living Cells IV
Daniel L. Farkas; Robert C. Leif; Robert C. Leif, Editor(s)

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