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Proceedings Paper

Electrical and optical methods for the manipulation and analyses of single cells
Author(s): Daniel T. Chiu; Maximilian Davidson; Anette Stroemberg; Frida Ryttsen; Owe Orwar
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Paper Abstract

This paper describes the use of focused electric fields and focused optical fields for the high-resolution manipulation of single cells. A focused electric field, obtained with the use of ultramicroelectrodes (tip diameter approximately 5 μm), is used to electroporate and electrofuse individual cells selectively and with high spatial resolution. A focused optical field, in the form of an optical tweezer, is used to isolate single organelles from a cell as well as to position liposomes incorporated with receptors and transporters along the cell for the high-resolution sampling and probing of the cellular microenvironment.

Paper Details

Date Published: 22 May 2001
PDF: 8 pages
Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); doi: 10.1117/12.426752
Show Author Affiliations
Daniel T. Chiu, Univ. of Washington (United States)
Maximilian Davidson, Goteborg Univ. (Sweden)
Anette Stroemberg, Goteborg Univ. (Sweden)
Frida Ryttsen, Goteborg Univ. (Sweden)
Owe Orwar, Goteborg Univ. (Sweden)

Published in SPIE Proceedings Vol. 4255:
Clinical Diagnostic Systems
Gerald E. Cohn, Editor(s)

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