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Proceedings Paper

Immunoaffinity-based biosensor for polycyclic aromatic hydrocarbons
Author(s): C. Brent Bargeron; Richard C. Benson; Terry E. Phillips; Peter F. Scholl; Sala Abubaker; John D. Groopman; Paul T. Strickland
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Paper Abstract

An automated biosensor for 1-hydroxypyrene-glucuronide (OHPG) has been developed using a sensor platform initially designed for aflatoxin. The platform is based on the properties of immunoaffinity for sample purification and concentration, and fluorescence for detection. Experiments have demonstrated capture, wash, elution, and quantitation with very good results. The device is handheld and battery- driven, and can be miniaturized further. The bed volume of the column was 0.1 ml with a capacity of about 400 ng of the OHPG. Analysis time was 10 - 11 minutes. The sensitivity was about 0.5 ppb. The antibodies for the system were developed previously and recognize OHPG. The biosensor relies upon microprocessor-controlled minifluidics and fluorometry.

Paper Details

Date Published: 22 May 2001
PDF: 7 pages
Proc. SPIE 4255, Clinical Diagnostic Systems, (22 May 2001); doi: 10.1117/12.426743
Show Author Affiliations
C. Brent Bargeron, Johns Hopkins Univ. (United States)
Richard C. Benson, Johns Hopkins Univ. (United States)
Terry E. Phillips, Johns Hopkins Univ. (United States)
Peter F. Scholl, Johns Hopkins Univ. (United States)
Sala Abubaker, Johns Hopkins Univ. (United States)
John D. Groopman, Johns Hopkins Univ. (United States)
Paul T. Strickland, Johns Hopkins Univ. (United States)

Published in SPIE Proceedings Vol. 4255:
Clinical Diagnostic Systems
Gerald E. Cohn, Editor(s)

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