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Proceedings Paper

Time-resolved UV-excited microarray reader for fluorescence energy transfer (FRET) measurements
Author(s): Adelina Orellana; Ari Petteri Hokkanen; Tomi Pastinen; Kristina Takkinen; Hans Soderlund
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Paper Abstract

Analytical systems based on immunochemistry are largely used in medical diagnostics and in biotechnology. There is a significant pressure to develop the present assay formats to become easier to use, faster, and less reagent consuming. Further developments towards high density array--like multianalyte measurement systems would be valuable. To this aim we have studied the applicability of fluorescence resonance energy transfer and time-resolved fluorescence resonance energy transfer in immunoassays on microspots and in microwells. We have used engineered recombinant antibodies detecting the pentameric protein CRP as a model analyte system, and tested different assay formats. We describe also the construction of a time-resolved scanning epifluorometer with which we could measure the FRET interaction between the slow fluorescence decay from europium chelates and its energy transfer to the rapidly decaying fluorophore Cy5.

Paper Details

Date Published: 10 May 2001
PDF: 9 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426728
Show Author Affiliations
Adelina Orellana, VTT Biotechnology and OrionPharma (Finland)
Ari Petteri Hokkanen, VTT Electronics (Finland)
Tomi Pastinen, McGill Univ. Health Ctr. (Finland)
Kristina Takkinen, VTT Biotechnology (Finland)
Hans Soderlund, VTT Biotechnology (Finland)

Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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