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Proceedings Paper

Dual-laser fluorescence correlation spectroscopy as a biophysical probe of binding interactions: evaluation of new red fluorescent dyes
Author(s): Kerry M. Swift; Steven Anderson; Edmund D. Matayoshi
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Paper Abstract

In this work we characterized seven dyes that are one-photon excited and fluoresce in the far-red region of the visible spectrum, five of which became available only recently, as to their suitability as labels on the red emission channel in a two-color FSC method. Spectroscopic properties and binding to albumin were studied. In addition, when one of these was used as the label in a first-pass high-throughput screen with single-color fluorescence polarization detection, its foreseen advantage of avoiding the excitation of interfering background autofluorescence from compounds in libraries was deemed valuable after a comparison of two complete high-throughput screens of a chemical compound library at Abbott.

Paper Details

Date Published: 10 May 2001
PDF: 12 pages
Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426726
Show Author Affiliations
Kerry M. Swift, Abbott Labs. (United States)
Steven Anderson, Abbott Labs. (United States)
Edmund D. Matayoshi, Abbott Labs. (United States)

Published in SPIE Proceedings Vol. 4252:
Advances in Fluorescence Sensing Technology V
Joseph R. Lakowicz; Richard B. Thompson, Editor(s)

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