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Proceedings Paper

Two-photon lifetime imaging of blood and blood vessels
Author(s): Cees J. de Grauw; Marc M.J. van Zandvoort; M. G.A. oude Egbrink; Dick W. Slaaf; Hans C. Gerritsen
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Paper Abstract

We investigated the potential of two-photon excitation microscopy for the imaging in large blood vessels. Experiments were carried out on isolated rat aorta, labeled with a DNA/RNA dye. Images of the vessel wall indicated that a penetration depth of more than 200 micrometers could be reached. Moreover, blood cells and platelets inside blood vessels could be imaged through the vessel wall. Fluorescence Lifetime Imaging (FLIM) was used as a contrast mechanism for discrimination of autofluorescence from fluorescence of labeled blood cells. We were able to observe labeled blood cells through the vessel wall and identify them by their morphology and characteristic fluorescent lifetimes.

Paper Details

Date Published: 24 April 2001
PDF: 6 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424551
Show Author Affiliations
Cees J. de Grauw, Utrecht Univ. (Netherlands)
Marc M.J. van Zandvoort, Univ. Maastricht (Netherlands)
M. G.A. oude Egbrink, Univ. Maastricht (Netherlands)
Dick W. Slaaf, Univ. Maastricht (Netherlands)
Hans C. Gerritsen, Utrecht Univ. (Netherlands)

Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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