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Proceedings Paper

Multiphoton microscopy: a retrospective focused on the visualization of embryo division dynamics
Author(s): David L. Wokosin
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Paper Abstract

The development of laser scanning fluorescence microscopy will be outlined. The focus will be technical instrumentation applied to solve biological problems through dynamic, high- resolution imaging. Laser scanning confocal microscopy will be presented first, followed by two-photon excitation fluorescence microscopy. Ideal imaging modes for two-photon imaging will be highlighted: deep tissue imaging and live cell imaging. Contributions from selected pioneers over the last decade of multi-photon imaging field will be highlighted in specific biological applications areas where two-photon imaging has already been established as the best (or only) option: intact tissues, developing embryos, and whole animal studies. The specific, unifying thread will focus in the quest for the observation of microtubule dynamics during the first few, asymmetric cell divisions in Caenorhabditis elegans embryos.

Paper Details

Date Published: 24 April 2001
PDF: 8 pages
Proc. SPIE 4262, Multiphoton Microscopy in the Biomedical Sciences, (24 April 2001); doi: 10.1117/12.424544
Show Author Affiliations
David L. Wokosin, Univ. of Strathclyde (United Kingdom)

Published in SPIE Proceedings Vol. 4262:
Multiphoton Microscopy in the Biomedical Sciences
Ammasi Periasamy; Peter T. C. So, Editor(s)

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