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Proceedings Paper

Application of fluorescence spectroscopy for on-line bioprocess monitoring and control
Author(s): Daniela Boehl; D. Solle; Hans Josef Toussaint; M. Menge; G. Renemann; Carsten Lindemann; Bernd Hitzmann; Thomas-Helmut Scheper
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Paper Abstract

12 Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy for data acquisition and the use of chemometric methods accomplish these requirements. The presented investigations were performed with fluorescence spectrometers with wide ranges of excitation and emission wavelength. By detection of several biogenic fluorophors (amino acids, coenzymes and vitamins) a large amount of information about the state of the bioprocess are obtained. For the evaluation of the process variables partial least squares regression is used. This technique was applied to several bioprocesses: the production of ergotamine by Claviceps purpurea, the production of t-PA (tissue plasminogen activator) by animal cells and brewing processes. The main point of monitoring the brewing processes was to determine the process variables cell count and extract concentration.

Paper Details

Date Published: 13 February 2001
PDF: 8 pages
Proc. SPIE 4201, Optical Methods for Industrial Processes, (13 February 2001); doi: 10.1117/12.417395
Show Author Affiliations
Daniela Boehl, Univ. Hannover (Germany)
D. Solle, Univ. Hannover (Germany)
Hans Josef Toussaint, Gilde Brauerei AG (Germany)
M. Menge, Univ. Hannover (Germany)
G. Renemann, Univ. Hannover (Germany)
Carsten Lindemann, DELTA Light & Optics (Denmark)
Bernd Hitzmann, Univ. Hannover (Germany)
Thomas-Helmut Scheper, Univ. Hannover (Germany)

Published in SPIE Proceedings Vol. 4201:
Optical Methods for Industrial Processes
Stuart Farquharson, Editor(s)

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