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Proceedings Paper

Effects of surface modification on placement and adhesion of NG108-15 cells
Author(s): Thomas W. Schneider; Heather M. Schessler; Kara M. Sheffer; Judy Dumm; L. E. Aloi
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Paper Abstract

A graded height flow cell was used to measure the adhesion properties of the neural cell line of neuroblastoma X glioma (NG108-15) cells cultured on substrates of organosilane self-assembled monolayers (SAMs). The SAMs tested in this study were 13F, 15F, PEG 550, OTS, DETA and APTS. Utilizing deep UV lithography, patterning of the SAMs create three regions for cell attachment; the original SAM, the backfilled SAM, and the interface between the two. Upon plating, the cell soma show no preference for any of the three regions. One exception was on PEG 550, which was found to resist cell adhesion upon normal plating conditions. The cell processes of the NG108-15 cells show a preference for growth at the interface between two patterned surfaces. A factor of three increase in adhesion properties was found for the patterned surface over an uncoated glass surface. Design rules of a single whole cell biosensor using theNG108-15 cells can be developed based on these findings.

Paper Details

Date Published: 29 December 2000
PDF: 10 pages
Proc. SPIE 4200, Biochemical and Biomolecular Sensing, (29 December 2000); doi: 10.1117/12.411719
Show Author Affiliations
Thomas W. Schneider, Science Applications International Corp. (United States)
Heather M. Schessler, Science Applications International Corp. (United States)
Kara M. Sheffer, Science Applications International Corp. (United States)
Judy Dumm, Science Applications International Corp. (United States)
L. E. Aloi, Science Applications International Corp. (United States)

Published in SPIE Proceedings Vol. 4200:
Biochemical and Biomolecular Sensing
Robert A. Lieberman, Editor(s)

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