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Proceedings Paper

Registration of immunoglobuline AB/AG reaction with planar polarization interferometer
Author(s): Alexei V. Nabok; Nickolaj F. Starodub; Asim K. Ray; Aseel K. Hassan
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Paper Abstract

Immobilization of human immunoglobuline (IgG) (AG) and goat on human IGG antibodies (AB) as well as AB/AG specific reaction were studied with planar polarization interferometry (PPI). In this novel method, polarized laser beam was coupled into the planar waveguide made on silicon wafer and consisted of 20nm Si3N4 layer sandwiched between two 1.5 micrometers SiO2 layers with the sensing window etched in the top SIO2 layer. One of the immune components was deposited by means of polyelectrolyte self- assembly on top of the Si3N3 layer within the sensing window, P-component of the polarized light is sensitive to adsorption, while s-component serves as a reference. Thus the outcoming light intensity depends on the phase shift between s- and p-components. Different sequences of immobilization of the immune components were studied with both surface plasmon resonance (SPR) and PPI methods. It was shown that predeposition of a monolayer of protein A, which is believed to affect the orientation of the immune components, causes an additional increase in the sensitivity. PPI method allowed us to improve substantially the sensitivity towards AB/AG reaction as compared to traditional SPR method. Particularly, of specific binding of 3ng/ml AG was registered.

Paper Details

Date Published: 29 December 2000
PDF: 9 pages
Proc. SPIE 4200, Biochemical and Biomolecular Sensing, (29 December 2000); doi: 10.1117/12.411710
Show Author Affiliations
Alexei V. Nabok, Sheffield Hallam Univ. (United Kingdom)
Nickolaj F. Starodub, A.V. Palladin Institute of Biochemistry (Ukraine)
Asim K. Ray, Sheffield Hallam Univ. (United Kingdom)
Aseel K. Hassan, Sheffield Hallam Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 4200:
Biochemical and Biomolecular Sensing
Robert A. Lieberman, Editor(s)

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