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Proceedings Paper

Observation of biological objects using an optical diffraction tomographic microscope
Author(s): Vincent Lauer
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Paper Abstract

A prototype of a tomographic microscope has bene realized, which uses phase-shifting holography to sense the wave diffracted by an object. The observed object is successively illuminated by a series of about 1000 plane waves. Each diffracted wave yields a part of the frequency representation of the object, and superposition of these parts yields a 3D frequency representation. An inverse Fourier transform yields the 3D spatial representation, from which sections, projections or stereoscopic images can be extracted. This prototype has been used to image a variety of biological samples. Images show a resolution limit of about a quarter of a wavelength and a depth of field of about 40 microns. Important factors for the understanding of image characteristics include horizontal and vertical resolution, ability to image horizontal and/or vertical surfaces, ability to distinguish variations of refractive index from variations of absorptivity. These and other imaging characteristics of the tomographic microscope are discussed on the basis of a set of images of biological samples. The connection between characteristics of a three-dimensional image and its frequency representation is explained. Influence of an object's characteristics (thickness, refractive index, ...) on image quality is described. Possible improvements and their impact on image quality is also discussed.

Paper Details

Date Published: 18 December 2000
PDF: 12 pages
Proc. SPIE 4164, Laser Microscopy, (18 December 2000); doi: 10.1117/12.410638
Show Author Affiliations
Vincent Lauer, Lauer Optique et Traitement du Signal (France)

Published in SPIE Proceedings Vol. 4164:
Laser Microscopy
Karsten Koenig; Hans J. Tanke; Herbert Schneckenburger, Editor(s)

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