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Proceedings Paper

Two-photon fluorescence microscopy of in-vivo human skin
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Paper Abstract

We present images of human skin obtained by a two-photon fluorescence microscope, which has been optimized for in- vivo imaging. Using autofluorescence as a contrast mechanism various layers of the skin, e.g., stratum corneum, viable epidermis, basal layer and upper dermis could be clearly distinguished. A comparison between two-photon fluorescence images and reflectance images shows that there is a clear difference in the information content provided by both techniques. In particular, the fluorescence images of the dermis show detail that is absent in the reflectance images.

Paper Details

Date Published: 18 December 2000
PDF: 6 pages
Proc. SPIE 4164, Laser Microscopy, (18 December 2000); doi: 10.1117/12.410637
Show Author Affiliations
Rob F. M. Hendriks, Philips Research Labs. (Netherlands)
Gerald W. Lucassen, Philips Research Labs. (Netherlands)

Published in SPIE Proceedings Vol. 4164:
Laser Microscopy
Karsten Koenig; Hans J. Tanke; Herbert Schneckenburger, Editor(s)

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