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Proceedings Paper

Kinetics of the accumulation of aluminum(III)-sulfophthalocyanine by human leukocytes measured with a scanning flow cytometer
Author(s): I. G. Scribunov; Pit A. Tarasov; K. A. Semianov; Valerii Pavlovich Maltsev; A. V. Chernyshev; E. R. Chernych; M. V. Tichonova; C. D. Nikonov
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Paper Abstract

The kinetics of aluminum (III)-sulfophthalocyanine uptake by human leukocytes was measured with a scanning flow cytometer (SFC) during the initial period of accumulation, 40 min. The individual cells were distinguished by SFC from their light scattering traces. The dye fluorescence in the cells was excited by N2 pulse laser, and the kinetics of the cell distribution on the amount of the accumulated dye was obtained. A mathematical model of endocytosis was applied in order to describe the dynamics of cell distribution in the system during the cellular uptake. The main kinetic parameters of the dye accumulation were evaluated.

Paper Details

Date Published: 3 November 2000
PDF: 12 pages
Proc. SPIE 4162, Controlling Tissue Optical Properties: Applications in Clinical Study, (3 November 2000); doi: 10.1117/12.405930
Show Author Affiliations
I. G. Scribunov, Institute of Chemical Kinetics and Combustion (Russia)
Pit A. Tarasov, Institute of Chemical Kinetics and Combustion (Russia)
K. A. Semianov, Institute of Chemical Kinetics and Combustion (Russia)
Valerii Pavlovich Maltsev, Institute of Chemical Kinetics and Combustion (Russia)
A. V. Chernyshev, Institute of Chemical Kinetics and Combustion (Russia)
E. R. Chernych, Institute of Clinical Immunology (Russia)
M. V. Tichonova, Institute of Clinical Immunology (Russia)
C. D. Nikonov, Siberian Ctr. of Laser Medicine (Russia)


Published in SPIE Proceedings Vol. 4162:
Controlling Tissue Optical Properties: Applications in Clinical Study
Valery V. Tuchin, Editor(s)

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