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Proceedings Paper

Water content and water profiles in skin measured by FTIR and Raman spectroscopy
Author(s): Gerald W. Lucassen; Peter J. Caspers; Gerwin J. Puppels
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Paper Abstract

We present non-invasive measurements of water content in stratum corneum (SC) by FTIR spectroscopy and water content profiles across the epidermis by Raman microspectroscopy. We apply band fitting to the FTIR spectra to assess changes in SC water content following hydration. While the penetration depth of ATR-FTIR is only a few micrometers, the confocal Raman microspectrometer enables successive collection of Raman spectra at a range of depths below skin surface, with an axial resolution of ca. 5micrometers . Depth resolved water concentration measurements of the epidermis obtained by Raman spectroscopy show clear changes in the water concentration profile as a result of hydration.

Paper Details

Date Published: 3 November 2000
PDF: 7 pages
Proc. SPIE 4162, Controlling Tissue Optical Properties: Applications in Clinical Study, (3 November 2000); doi: 10.1117/12.405921
Show Author Affiliations
Gerald W. Lucassen, Philips Research Labs. (Netherlands)
Peter J. Caspers, Erasmus Univ. Rotterdam (Netherlands)
Gerwin J. Puppels, Erasmus Univ. Rotterdam (Netherlands)

Published in SPIE Proceedings Vol. 4162:
Controlling Tissue Optical Properties: Applications in Clinical Study
Valery V. Tuchin, Editor(s)

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