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Proceedings Paper

Effect of He-Ne laser irradiation on erythrocyte and lymphocyte membranes of children in vitro as studied by the intrinsic and extrinsic fluorescence techniques
Author(s): Anna V. Volotovskaya; Nataly M. Kozlova; Ekaterina I. Slobozhanina; Vladimir S. Ulaschik; Vasili A. Mostovnikov
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Paper Abstract

In recent years the treatment of blood with low intensity laser irradiation has become popular in a variety of clinical applications due to its anti-inflammatory, biostimulative and immune-stimulatory effects etc. Despite of wide using of laser blood irradiation in the pediatric practice there is lack of information concerning the sensitivity of children blood cells to laser irradiation. At present study the influence of the He-Ne laser irradiation on the lipid physico-chemical state in lymphocytes and isolated erythrocyte membranes of 8-16 years old children using lipophilic fluorescence probe pyrene was investigated in vitro. It was shown that fluorescence parameters of pyrene incorporated into erythrocyte and lymphocyte membranes after laser irradiation ((lambda) equals 630nm) at dose of 24 J/cm2 at t equals 18 +/- 2 (degree)C were unchanged. The intensity of intrinsic protein UV-fluorescence ((lambda)ex equals 297 nm, (lambda)em equals 332 nm) of lymphocytes exposed to the same irradiation was decreased insignificantly. The obtained data indicate that He-Ne laser irradiation at the above dose does not affect the lipid microviscosity of erythrocyte and lymphocyte membranes.

Paper Details

Date Published: 3 November 2000
PDF: 5 pages
Proc. SPIE 4159, Effects of Low-Power Light on Biological Systems V, (3 November 2000); doi: 10.1117/12.405914
Show Author Affiliations
Anna V. Volotovskaya, Belarussian State Advanced Doctor's Training Institute (Belarus)
Nataly M. Kozlova, Institute of Photobiology (Belarus)
Ekaterina I. Slobozhanina, Institute of Photobiology (Belarus)
Vladimir S. Ulaschik, Belarussian State Advanced Doctor's Training Institute (Belarus)
Vasili A. Mostovnikov, Institute of General Physics (Belarus)

Published in SPIE Proceedings Vol. 4159:
Effects of Low-Power Light on Biological Systems V
Tiina I. Karu; Rachel Lubart, Editor(s)

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