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Proceedings Paper

Experimental research of photodynamic diagnosis mediated by chemiluminescence reagents
Author(s): Juan Wang; Da Xing; Yonghong Tang; Yonghong He
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Paper Abstract

There are some serious limitations existing in conventional photodynamic diagnosis (PDD), such as autofluorescence disturbance, weak red-band absorption efficacy of traditional photosensitizers and quite short penetration depth of excitation light. In this paper a novel method of PDD mediated by chemiluminescence reagents is presented in fabricated mixed samples. Photodynamic therapy (PDT) is believed to act via singlet oxygen (1O2) generated by energy from photosensitizers. 5-Fluoresceinyl Cypridina Luciferin Analog (FCLA), a chemiluminescence reagent, can react with singlet oxygen and emit chemiluminescence with peak wavelength about at 526 nm. In the experiments, FCLA was chosen as an optical reporter of localization of singlet oxygen produced from photosensitized reaction of Hematoporphyrin Derivative (HpD) excited by 630 nm laser. Experimental results, the measurement of fluorescence spectra and the detection of image from chemiluminescence, show that the mixed HpD and FCLA in biologic tissue mixture excited with the laser of 630 nm can emit detectable light signals.

Paper Details

Date Published: 11 October 2000
PDF: 5 pages
Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000);
Show Author Affiliations
Juan Wang, South China Normal Univ. and Anhui Institute of Optics and Fine Mechanics (China)
Da Xing, South China Normal Univ. (China)
Yonghong Tang, South China Normal Univ. (China)
Yonghong He, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 4224:
Biomedical Photonics and Optoelectronic Imaging
Hong Liu; Qingming Luo, Editor(s)

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