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Proceedings Paper • Open Access

From microfluidics to nanofluidics: DNA separation using nanofluidic entropic trap array device
Author(s): Jongyoon Han; Harold G. Craighead

Paper Abstract

Fluidic devices with sub-micrometer dimensions provide new opportunities in manipulation and analysis of various biomolecules, such as deoxyribonucleic acid (DNA). As an example of such devices, a microchannel with an array of entropic traps is introduced. The existence of sub-100nm constriction causes long double-stranded DNA molecules to be entropically trapped, and the length-dependent escape of DNA from the trap enables a band separation of DNA. Entropic traps are also used to manipulate and collect many DNA molecules into a narrow, well-defined initial band for electrophoresis launching. In addition to its speed and compactness, another important advantage of this artifical separation device over conventional gel electrophoresis is the ability to modify and control the device precisely for the optimization of a separation process. The similar device could be used to analyze proteins or other biopolymers.

Paper Details

Date Published: 18 August 2000
PDF: 7 pages
Proc. SPIE 4177, Microfluidic Devices and Systems III, (18 August 2000); doi: 10.1117/12.395654
Show Author Affiliations
Jongyoon Han, Cornell Univ. (United States)
Harold G. Craighead, Cornell Univ. (United States)

Published in SPIE Proceedings Vol. 4177:
Microfluidic Devices and Systems III
Carlos H. Mastrangelo; Holger Becker, Editor(s)

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