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Proceedings Paper

Application of phase contrast microscopy to the study of marine microbiota
Author(s): MingChang Shih; Chang Ten Lian; J. S. Hwang
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Paper Abstract

We present the observation of the activity of artemia, one of the popular marine micro-biota species, in free space by the application of Fourier optics imaging technique. The Fourier optic imaging system is consisted by a collimated laser beam source, a Fourier spatial filter, an non-coherent IR source, and a CCD imaging system. By recording the images of Artemia's motion in real life, we are able to study the fundamental patterns of artemia motion mechanism, and the response of the motion pattern to the variation of its environment. Characteristic patterns of artemia's motion, such as linear motion, spiral motion, and mating collision are observed. It is shown that the increasing of the environment temperature driving the motion of the artemia's moving faster and more frequently, and still stays alive even at the environment temperature up to 38 C.

Paper Details

Date Published: 4 July 2000
PDF: 5 pages
Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390552
Show Author Affiliations
MingChang Shih, National Taiwan Ocean Univ. (Taiwan)
Chang Ten Lian, National Taiwan Ocean Univ. (Taiwan)
J. S. Hwang, National Taiwan Ocean Univ. (Taiwan)

Published in SPIE Proceedings Vol. 4082:
Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications
Robert R. Alfano; Peng Pei Ho; Arthur E. T. Chiou, Editor(s)

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