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Proceedings Paper

Applications of optical tweezers and an integrated force measurement module for biomedical research
Author(s): Jin-Wu Tsai; Bing-Yao Liao; Chun-Cheng Huang; Wen-Liang Hwang; Da-Wei Wang; Arthur E. T. Chiou; Chi-Hung Lin
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Paper Abstract

Optical tweezers are useful for manipulating biological samples and measuring biological forces. In the present study, we have integrated a forward scatter analysis (FORSA) module in the single-beam gradient force optical tweezers. The entire set-up was then incorporated onto an inverted microscope. In the FORSA module an Helium-Neon probing laser was spotted (at a slightly out-of-focus way) onto the object being trapped by the infrared laser-based tweezers and generated a diffraction pattern. Imagines of the diffraction pattern were captured by a charge- coupled device (CCD), and digitized and processed by a computer. Wed demonstrated that tracking the amplified diffraction pattern war much more precise to determine the movement of the object within the trap than analyzing the minute motion of the object itself. Displacement of the object could then be translated into the force being applied by the tweezers. Also, using an algorithm developed in the lab, we were able to follow the movement of the scattering pattern at a temporal resolution close to video rate. We have used this system to investigate the binding force associate with cell-cell interactions and modular interactions. In these studies. A cell was carefully positioned to make contact with another cell or a microparticle coated with proteins of interest by optical tweezers in a well-controlled manner. During these events, we noted a progressive increase of cell adhesion at the immediate early period (i.e., a few minutes after initial contact) of cell-cell interactions. Also, binding of a disintegrin, rhodostomin, and its mutant to the counterpart integrin on the cell surface could be assessed with great convenience and accuracy. Our results demonstrated that addition of the forward scatter analysis module to convention optical tweezers provides an effective and convenient way for monitoring biological activities in situ and measuring changes of biological forces with precision.

Paper Details

Date Published: 4 July 2000
PDF: 9 pages
Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390549
Show Author Affiliations
Jin-Wu Tsai, National Yang-Ming Univ. (Taiwan)
Bing-Yao Liao, Institute of Information Science (Taiwan)
Chun-Cheng Huang, National Dong Hwa Univ. (Taiwan)
Wen-Liang Hwang, Institute of Information Science (Taiwan)
Da-Wei Wang, Institute of Information Science (Taiwan)
Arthur E. T. Chiou, National Dong Hwa Univ. (Taiwan)
Chi-Hung Lin, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 4082:
Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications
Robert R. Alfano; Peng Pei Ho; Arthur E. T. Chiou, Editor(s)

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