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Proceedings Paper

Multiphoton fluorescence microscopy: behavior of biological specimens under high-intensity illumination
Author(s): Ping Chin Cheng; Bai-Ling Lin; Fu-Jen Kao; Chi-Kuang Sun
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Paper Abstract

Recent development in multi-photon fluorescence microscopy, second and third harmonic generation microscopy (SHG and THG) and CARS open new dimensions in biological studies. Not only the technologies allow probing the biological specimen both functionally and structurally with increasing spatial and temporal resolution, but also raise the interest in how biological specimens respond to high intensity illumination commonly used in these types of microscopy. We have used maize leaf protoplast as a model system to evaluate the photo-induced response of living sample under high intensity illumination. It was found that cells can be seriously damaged by high intensity NIR irradiation even the linear absorption coefficient in low in these wavelengths. Micro-spectroscopy of single chloroplast also allows us to gain insight on the possible photo-damage mechanism. In addition to fluorescence emission, second harmonic generation was observed in the maize protoplasts.

Paper Details

Date Published: 4 July 2000
PDF: 5 pages
Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390536
Show Author Affiliations
Ping Chin Cheng, SUNY/Buffalo (United States)
Bai-Ling Lin, Institute of Molecular Biology (Taiwan)
Fu-Jen Kao, National Sun Yat-sen Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 4082:
Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications
Robert R. Alfano; Peng Pei Ho; Arthur E. T. Chiou, Editor(s)

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