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Proceedings Paper

Human cell viability to laser pulse and ion transport processes
Author(s): Dmitry Lapotko; Tat'yana Romanovskaya
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Paper Abstract

We have studied experimentally the problem of cell viability during laser-tissue interaction: how the cell viability can be regulated under certain laser regime. Many modern laser therapeutic techniques assume that cells should survive laser therapy or even to become activated (laser stimulation of immune system). An influence of ion balance was studied for human Red Blood Cells (RBC) during in vitro measurements of single RBC viability after laser pulse. Single cell viability was measured optically with Laser Viability Test which is based upon interpretation of cell photothermal response to pump pulse (532 nm, 10 ns, 10 - 50 (mu) J at 12 micrometer diameter). According to our experiments laser-induced cell damage occurs at different laser energy levels within one cell population (heterogeneity of cell viability) and besides is donor-dependent. The latter result means that laser therapeutic dose should be adjusted individually. For solutions of the ions Na+, K+, Ca++ and Mg++ we have found strong dependence of cell viability upon the process of ion transport through the cell membrane which was regulated by variation of concentration and composition of above mentioned ions. An increase of Na+ or K+ concentration causes a decrease of RBC laser viability.

Paper Details

Date Published: 13 June 2000
PDF: 8 pages
Proc. SPIE 3914, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, (13 June 2000); doi: 10.1117/12.388070
Show Author Affiliations
Dmitry Lapotko, A.V. Luikov Heat and Mass Transfer Institute (United States)
Tat'yana Romanovskaya, International Sakharov Environmental Univ. (Belarus)

Published in SPIE Proceedings Vol. 3914:
Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
Jeffrey O. Hollinger D.D.S.; Donald Dean Duncan; Jeffrey O. Hollinger D.D.S.; Donald Dean Duncan; Steven L. Jacques, Editor(s)

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